Antibacterial effect of Aqueous Neem (Azadirachta indica) leaf extract, crude neem leaf paste, and Ceftriaxone against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

Authors

  • Shamima Sultana Mymensingh Medical College
  • A. S. M. Shahidullah SSNIMC
  • Md. Mahbubul Islam Bangladesh Jute Research Institute
  • A. F. S. A. Wasey United Hospital
  • Shamsun Nahar Mymensingh Medical College

DOI:

https://doi.org/10.18034/mjmbr.v3i1.401

Keywords:

Antibacterial effect, crude neem leaf paste (CNLP), Aqueous neeem leaf extract (ANLE), Ceftraxione (antibiotic), Minimum inhibitory concentration (MIC), Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa

Abstract

The study was conducted during the period of July 2004 to June 2005 in the   Department of Pharmacology & Therapeutics in collaboration of Department of Microbiology, Mymensingh Medical College, Mymensingh to determine the profile of antibacterial effect of crude neem leaf paste (CNLP), aqueous neem leaf extract (ANLE), and standard antibiotic Ceftriaxone against Staphylococcus aureus, Escherichia coli and, Pseudomonas aeruginosa. Five separate experiments were done e.g. I) Determination of inhibitory effect of crude neem leaf paste (CNLP) by incorporation into nutrient agar media (NA), against Staphylococcus aureus, Escherichia coli and, Pseudomonas aeruginosa. II) Determination of minimum inhibitory concentration (MIC) of aqueous neem leaf extract (ANLE) against that three test organisms by broth dilution technique, III) Determination of minimum inhibitory concentration (MIC) of standard antibiotic ceftriaxcone against test organisms by broth dilution technique as well as making a comparison with MIC of ANLE and IV) Subculture study of materials from effective CNLP, ANLE, NLEE and Ceftriaxone in nutrient agar medium for confirmation of respective results of different experiments conducted. Results revealed that inhibitory effects were observed against the growth of Staph. aureus, Esch. coli and Ps. aeruginosa at 15%, 20% and 25% respectively of CNLP incorporated  into NA media.  The broth dilution technique was followed to determine the MICs of ANLE and Ceftriaxone. The MIC of ANLE was 714 μg/ml against S. aureus and that against E. coli and P. aeruginosa was 1428 μg/ml. The MIC of Ceftriaxone was 10μg/ml against S. aureus and that against E. coli and P. aeruginosa was 25 μg/ml. The MIC of Ceftriaxone was the lowest in comparison to MICs of ANLE. The subculture study showed similar results with that of previous experiments in terms of inhibitory effects of CNLP and MICs of ANLE, Ceftriaxone against all of the organisms studied.

 

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Author Biographies

  • Shamima Sultana, Mymensingh Medical College

    Associate Professor, Department of Pharmacology, Mymensingh Medical College, Mymensingh, BANGLADESH

  • A. S. M. Shahidullah, SSNIMC

    Assistant Professor, Department of Community Medicine, SSNIMC, Kishoregonj, BANGLADESH

  • Md. Mahbubul Islam, Bangladesh Jute Research Institute

    Chief Scientific Officer, Agronomy Division, Bangladesh Jute Research Institute, Manikmia Avenue, Dhaka, BANGLADESH

  • A. F. S. A. Wasey, United Hospital

    Consultant, Department of Microbiology/Clinical Pathology, United Hospital, Dhaka, BANGLADESH

  • Shamsun Nahar, Mymensingh Medical College

    Professor Pharmacology, Department of Pharmacology, Mymensingh Medical College, Mymensingh, BANGLADESH

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Published

2016-06-30

Issue

Vol. 3 No. 1 (2016): January - June Issue

Section

Peer-reviewed Article

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How to Cite

Sultana, S. ., Shahidullah, A. S. M. ., Islam, M. M. ., Wasey, A. F. S. A. ., & Nahar, S. . (2016). Antibacterial effect of Aqueous Neem (Azadirachta indica) leaf extract, crude neem leaf paste, and Ceftriaxone against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Malaysian Journal of Medical and Biological Research, 3(1), 25-36. https://doi.org/10.18034/mjmbr.v3i1.401
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